High-throughput imaging of dynamics of single cells and multi-cellular clusters.
Many biological processes in multi-cellular organisms are regulated by various dynamic cellular processes, but classical tissue culture system can only provide static environments where many dynamic events occur randomly, thus mechanistic study of cellular dynamics has been limited. To overcome this limitation, we developed a new experimental platform where we can precisely position different types of cells and control their movement by illuminating light using our bio-friendly photoresist PDMP .
By combining automated microscopy and image processing, we could systematically study the effect of various pharmacological inhibitors for cytoskeleton regulators on cell spreading . Also, multi-cellular clusters of small number of cells with precisely controlled number and composition were successfully fabricated . Currently, we are monitoring molecular activities (in collaboration with Prof. Yingxiao Wang at UC San Diego) and distribution of specific lipids (in collaboration with Prof. Wonhwa Cho at University of Illinois at Chicago) within cells using fluorescence-based biosensors during various dynamic cellular processes using this novel platform.
1. M. Kim, J.-C. Choi, H.-R. Jung, J.S. Katz, M.-G. Kim, and J. Doh*, Addressable micropatterning of multiple proteins and cells by microscope projection photolithography based on a protein friendly photoresist, Langmuir 26, 12112 (2010).
2. J.-C. Choi and J. Doh*, High-throughput quantitative imaging of cell spreading dynamics by multi-step microscopy projection photolithography based on a cell-friendly photoresist, Lab Chip 12, 4964 (2012).
3. J.-C. Choi, H.-R. Jung and J. Doh*, Dynamic modulation of multi-cellular clusters using a cell-friendly photoresist, (in preparation).